Biophysics & Soft Condensed Matter
The image shows the fluorescence emission from a lipid membrane with membrane-anchored polymers assembled on a terraced substrate that enables the use of "fluorescence interference contrast microscopy," a technique by which the interference of light from fluorophores with its reflection from a substrate can yield nanometer-scale topographic information. The colors are due to different interference patterns from emission from red lipid-conjugated probes and green polymer-conjugated probes. The image width is about 200 microns. Further information, including analysis of this data, can be found in our recent paper:
Kamil Godula, Marissa L. Umbel, David Rabuka, Zsofia Botyanszki, Carolyn R. Bertozzi, and Raghuveer Parthasarathy, "Control of the molecular orientation of membrane-anchored biomimetic glycopolymers," J. Am. Chem. Soc. 131: 10263-10268 (2009).
© 2010 -- Raghuveer Parthasarathy,
University of Oregon,
Department of Physics and Materials
Unauthorized duplication of images is strictly prohibited!
Contact: raghu [at] uoregon.edu
July 1, 2010